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  • Have you noticed the experimental techniques of ELISA?

    R&D Systems offers a wide range of Quantikine® ELISA kits that are fully tested to ensure superior quality and repeatability. This ready-to-use ELISA kit contains all the reagents and consumables required for ELISA experiments, and by carefully selecting antibody pairs and optimizing formulation dilutions to ensure unparalleled accuracy. We have also specifically introduced its features in previous articles.


    R&D Systems makes Quantikine® ELISA kits very powerful, even for inexperienced users. At the same time, we also provide some experience and skills to use, even experienced users can benefit from it. Are you looking forward to seeing it?

    01. Experimental preparation stage

    Before the start of the ELISA experiment, there are usually some reagents that need to be prepared in advance, and there are some details to be aware of:

    Before the start of the experiment, it is necessary to ensure that all reagents have reached room temperature before use (unless specified to keep the temperature low).

    If you are not planning to use a complete block (96 wells), be sure to put the remaining 8 tubes back into the desiccant plate and seal them to prevent the product from degrading.

    For standards that are not disposable, it is best to dispense the remaining standard in small quantities and freeze. This frees the standard from repeated freezing and thawin

    02, experimental operation stage

    In the ELISA experiment operation, in order to obtain higher and better results, in particular, you need to pay attention to some details of the loading:

    Multi-channel pipettes speed up the loading of your standards and samples and deliver consistent results.

    When pipetting, the tip is offset from the vertical by a slight angle, but do not touch the bottom of the sample well.

    When the sample of the double hole is loaded, it is not necessary to change the tip; however, in order to avoid sample contamination, the tip is changed when taking different samples or standards.


    03, wash plate and incubation stage

    During the wash and incubation phases, these suggestions may be helpful in improving your experimental results:

    It is highly recommended to use a washer, which may result in a higher background

    When washing the plate, whether by hand or with a plate washer, an infiltration time of 30 seconds is added between the two washes to ensure the washing effect.

    Carefully observe the incubation time. A general recommendation is to control the error of the incubation time per hour to within +/- 5 minutes.

    If the test requires incubation at a low temperature of 2-8 degrees and you are working on multiple plates at the same time, do not stack the plates on top of one another and place them side by side.


    R&D Systems, a Bio-Techne brand, has 25 years of experience in the design, testing and optimization of immunoassay kits to ensure the highest level of analyte quantification. We currently offer more than 600 complete, ready-to-use Quantikine® ELISA kits and more than 1,000 Duoset® ELISA development kits for the detection of different analytes including humans, mice, rats, dogs, Primates and pigs.


    Zhongyuan Bio is an authorized agent of R&D Company


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